Generasi Pertama (F1) Transfer Gen waxy (wx) dari Jagung Pulut ke Jagung Lokal Manokwari

Abstract

The objectives of this researchwas to transfer waxy gene that encodesamylopectin from waxy corn to severalManokwari-local corns. The study was designed using a single row. There weresix genotypes of local corn as female parent planted in a single row and they were flanked by two rows of waxy corn served as male parent. The hybridization method used was cross-pollination method. The present of waxy gene(wx) in the corn endosperm which is the generation of crossbred was detected using iodine-color method. The characteristics of generative of F1 were still heterogeneous, both in population and among population of genotype ofManokwari-local corns. The color seed produced from the cross of six genotypes oflocal corn with waxy corn was vary, especially the F1 population of Anggi-purple genotype. However, some cobs of F1 had uniform color already. The amylopectin content inF1 of the genotype of Manokwari-local corn was low,indicated by the endosperm which was still purple. Therefore, it is necessary to carry out again a transfer of waxy gene from waxy corn to F1.