Abstract:
Marine organisms have developed exquisitely complex biological mechanisms and they are known as source producing bioactive compound. The objective of this study is to test cell-free P. aeruginosa NS3 on pathogenic microbes and to identify antimicrobial compounds in supernatants. The method bacterial culture of P. aeruginosa NS3 was harvested on 72hour at the exponential growth phase and it was then centrifuged. Cell-free supernatants were tested on pathogenic microbes using disc diffusion method, the pigment components were characterized by UV-vis and weightmolecules of bioactive secondary metabolites was identified using LC-MS/MS. The result showed that cell-free supernatants of P. Aeruginosa NS3 could inhibit pathogenic microbes with MRSA inhibition zone of 8 ± 0.5 mm, S. aureus 25 ± 2.7 mm, EPEC30 ± 5.2 mm and Candida albicans 22 ± 3.5 mm. The minimum inhibitory concentration ranged from 31.25 to 500 ppm. The P. aeruginosaNS3 bacteria produced phenazine antimicrobial compounds with pyocyanin component (211m/z), 1-hydroxyphenazine (196m/z), phenazine-1-carboxamide (179m/z) and phenazine-1- carboxylic acid (155 m/z).P. aeruginosaNS3 bacteria producedphenazine-containing compound that has the ability to inhibit pathogenic microbes. The greatest inhibitors were EPEC, S. aureus, C. albicans and MRSA. It is necessary to conduct further research which uses their bioactive compounds in pharmaceuticals such as antiviral, anticancer, and antiinflammatory
