Abstract:
This study aimed to identify the genotype of the gene Pituitary-specific transcription factor 1 (Pit-1) in Bali cattle (Bos sondaicus). A total of 20 Bali cattle (13 males and seven females) were used in this study. DNA samples were isolated from blood samples using the phenol-chloroform extraction method. This study uses the PCR-RFLP method. Amplification of specific DNA fragments (size 451 bp, intron regions are stretching from 4 to exon 6) of the Pit-1 gene Bali cattle samples was performed using a pair of specific primers (forward: 5'-AAA-CCA-TCATCT-CCC-TTC-TT -3 ', and reverse: 5'-AAT-GTA-CAA-TGT-GCC-TTC-TGA-G-3'). Based on the RFLP analysis/HinfI, all Bali cattle research resulted in a uniform (monomorphic) band pattern. The band sizes were 244 bp and 207 bp, and the band patterns were thus identified as genotype BB, so it is known that the frequency of the A and B alleles is at 0 and 1. The frequency of the A allele was not found in Bali cattle (Bos sondaicus) and showed a significant difference with other cattle breeds (Bos taurus and Bos indicus).
