In vivo Efficacy of Biophytum petersianum on Haemonchus contortus in Goats

Abstract

Haemonchosis is a common and severe disease caused by the infection of worms Haemonchus sp. Kebar grass (Biophytum petersianum) known as a plant from Papua Indonesia that contains tannin compounds and has potential anthelmintic activity. The present study was carried out to determine the influence of Kebar grass infusion on H. contortus under in vivo conditions. A total of 15 female goats at 6-8 months old were divided into 5 groups, and each consisted of 3 goats. All groups were infected 1000 infective larvae orally every week for 4 weeks. Three groups were given Kebar grass infusion a day at a dose of 2 mg/mL, 4 mg/mL and 6 mg/mL respectively for 7 days at the 6th week. Group 4 was given Albendazole at a dose of 3.8 mg/kg body weight, and group 5 was not given treatment. Clinical examinations, hematological and EPG were carried out every week. All goats were autopsied and analyzed for pathology at the seventh week. Quantitative data to perform statistical analysis, as well as the results of physical examination and histopathology were analyzed descriptively. Body weight measurements in vivo as the mean, Body Condition Scores (BCS), and FAMACHA showed an increase. RBC, Hb, PCV and total protein values increased. The FECR value of the treatment group was higher than the negative control group. During the autopsy, both carcass and visceral organs were colourless, slight of subcutaneous fat, and small intestine haemorrhage and ascites in the abdominal cavity. Many nodules with diameters between 1.5-2.0 mm were observed in the abomasal mucosa. Histopathological changes in the control group were infiltration of inflammatory cells, congestion and the presence of worm pieces. Based on results obtained in this line of research, it is therefore concluded that Kebar grass infusion is anthelmintic against H. contortus under in vivo conditions.