Abstract:
Objective: This study was carried out to evaluate the nutritive value, in vitro fermentation characteristics and nutrient digestibility ofagro-industrial byproducts-based complete feed block enriched with mixed microbes. Methodology: The complete feed blocks weremainly composed of agricultural and food industry byproducts such as rice straw, palm oil frond, tofu waste, cassava waste, sago starchand molasses. Four treatments were A, complete feed block without microbe; B, complete feed block containing Lactobacillus plantarum,Saccharomyces cerevisiae and Pseudomonas aeruginosa; C, complete feed block containing L. plantarum, S. cerevisiae andAcinetobacter baumannii ; D, complete feed block containing L. plantarum, S. cerevisiae, P. aeruginosa and A. baumannii. Lactic acidbacteria, yeast and cellulolytic bacteria were added to the feed block at 107-109 CFU gG1. All complete feed blocks were formulated to beisonitrogenous. About 500 g of mixed ingredients were transferred into a hydraulic press to makes block of size 15×10×8 cm.Results: The feed blocks contained 2.4×106 CFU gG1L. plantarum, 6.5×104 CFU gG1, 2.7×106 CFU gG1S. cerevisiae, 2.7×106 CFU gG1P. aeruginosa and 1.4×105 CFU gG1A. baumannii. Crude protein content was similar (11.6%) for 4 feed blocks. Addition of cellulolyticbacteria in feed block reduced (p<0.01) Neutral Detergent Fiber (NDF) and Acid Detergent Fiber (ADF) contents. Feed blocks containingmixed microbes (C and D) had higher (p<0.01) acetic acid, propionic acid, butyric acid, total Volatile Fatty Acids (VFA) concentrations aswell as total gas production compared with feed block without microbe (A). Feed block containing combination of L. plantarum,S. cerevisiae, P. aeruginosa and A. baumannii had the highest Dry Matter (DM), Organic Matter (OM) and NDF digestibility.Conclusion: Combination of mixed microbes in the complete feed block decreased fiber fraction contents and improved in vitrofermentation activity and the nutrients digestibility.