Assessment of Genetic Arrangement of Sago palm Collection Based on Mitochondrial nad2 gene Marker

Abstract

Mitochondrial DNA is an organelle found in plants and animals that play a role in the formation of energy. NADH and ATP are forms of chemical energy which resulting by plants and animals for their growth and development. The genes that play a role in the biosynthesis of NADH and ATP sequentially are the nad and atp genes. Gent nad and atp are inherited maternally so it is very conservative. The aims of work are evaluated genetic characteristic of sago palm accessions based on nad-2 gene. Methods of research were DNA extraction by using Geneaid DNA plant mini Kit, PCR amplification fragments DNA by using KAPA 2G Robust Hotstar polymerases and perform by using Biorad PCR instrument, purifying and sequencing DNA by Macrogen Inc. Seoul, Korea. Data analyses were perform by using MEGA 6 software. Morphological characteristics in the russet stages of sago palm accessions were divided into four phenotypic and several accessions show phenotypically the same. Based on nad-2 gene was shown that the sago palm accessions have the same genetic constitution among the others. Phylogenetic constructions of sago palm accession and other related plant based on nad-2 gene markers were described that sago palm closer to the coconut and date palm and then following others plant producing large amount carbohydrates.